Journal: Chemical Senses

Article Title: Commensal Bacteria Regulate Gene Expression and Differentiation in Vertebrate Olfactory Systems Through Transcription Factor REST

doi: 10.1093/chemse/bjz050

Figure Lengend Snippet: Nasal commensal bacteria promote rat Odora cell differentiation in vitro in a REST dependent manner. (A) Undifferentiated Odora cells were incubated with the nasal bacterial strain S. aureus, the nasal bacterial strain C. denitrificans, or the gut commensal strain (A. radioresistens). OMP expression levels were measured 72 h later by RT-qPCR. Data are shown as the mean fold change ± SE in expression compared to undifferentiated controls. (B) Kinetics of OMP gene expression; (C) oCNGb gene expression; and (D) REST gene expression in undifferentiated Odora cells, control differentiated Odora cells, or Odora cells differentiated in the presence of live S. aureus at 6, 24, 48, and 72 hours. Data are shown as the mean fold change ± SE in expression compared to undifferentiated controls measured by RT-qPCR. Asterisks denote statistically significant differences (P < 0.05). Results are representative of 3 independent experiments with N = 5 per experiment.

Article Snippet: Comamonas denitrificans was purchased from American Type Culture Collection (ATCC 700940).

Techniques: Bacteria, Cell Differentiation, In Vitro, Incubation, Expressing, Quantitative RT-PCR, Gene Expression, Control